Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC10 MC100 A Total reads 7,261,424 7,261,424 7,261,424 B Total samples 119 119 119 C Samples with reads ≥ 500 reads (S≥500) 108 108 108 D Samples with reads < 500 reads (S<500) 11 11 11 E Reads in samples S≥500 7,261,179 7,261,179 7,261,179 F Reads in samples S<500 (removed) 245 245 245 G Total reads assigned 4,033,832 4,028,701 3,997,833 H Reads assigned to single species 1,267,262 1,266,349 1,260,969 I Reads assigned to multiple species 338,300 338,012 335,780 J Reads assigned to novel species 2,428,270 2,424,340 2,401,084 K Number of single species 580 275 115 L Number of multi-species 171 92 26 M Number of novel species 2,609 1,326 667 N Total reads unassigned 3,227,347 3,232,478 3,263,346 O Reads excluded (<MC) 0 5,131 35,999 P Chimeric reads 424,522 424,522 424,522 Q Reads without BLASTN hits 20,060 20,060 20,060 R Singleton reads 2,782,765 2,782,765 2,782,765 1. A=E+F A=F+G+N G=H+I+J N=O+P+Q+R 2. View all read count by samples 3. All data can be downloaded in a compressed ZIP file ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions