Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC2 MC5 MC10 MC50 MC100 A Total reads 54,970 54,970 54,970 54,970 54,970 54,970 B Total samples 11 11 11 11 11 11 C Samples with reads ≥ 500 reads (S≥500) 11 11 11 11 11 11 D Samples with reads < 500 reads (S<500) 0 0 0 0 0 0 E Reads in samples S≥500 54,970 54,970 54,970 54,970 54,970 54,970 F Reads in samples S<500 (removed) G Total reads assigned 52,925 52,804 52,554 52,140 49,092 46,605 H Reads assigned to single species 27,249 27,220 27,113 26,956 25,221 23,961 I Reads assigned to multiple species 6,449 6,442 6,435 6,416 6,292 6,019 J Reads assigned to novel species 19,227 19,142 19,006 18,768 17,579 16,625 K Number of single species 207 178 139 116 41 23 L Number of multi-species 28 21 18 15 10 6 M Number of novel species 257 172 123 89 31 18 N Total reads unassigned 2,045 2,166 2,416 2,830 5,878 8,365 O Reads excluded (<MC) 0 121 371 785 3,833 6,320 P Chimeric reads 2,044 2,044 2,044 2,044 2,044 2,044 Q Reads without BLASTN hits 1 1 1 1 1 1 R Singleton reads 0 0 0 0 0 0 1. A=E+F A=F+G+N G=H+I+J N=O+P+Q+R 2. View all read count by samples 3. All data can be downloaded in a compressed ZIP file ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions