Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC10 MC100 A Total reads 2,797,837 2,797,837 2,797,837 B Total assigned reads 2,270,978 2,270,978 2,270,978 C Assigned reads in species with read count < MC 0 1,837 13,816 D Assigned reads in samples with read count < 500 0 0 0 E Total samples 22 22 22 F Samples with reads >= 500 22 22 22 G Samples with reads < 500 0 0 0 H Total assigned reads used for analysis (B-C-D) 2,270,978 2,269,141 2,257,162 I Reads assigned to single species 1,155,885 1,155,315 1,148,669 J Reads assigned to multiple species 1,089,287 1,088,960 1,085,852 K Reads assigned to novel species 25,806 24,866 22,641 L Total number of species 1,139 573 248 M Number of single species 503 330 167 N Number of multi-species 237 145 63 O Number of novel species 399 98 18 P Total unassigned reads 526,859 526,859 526,859 Q Chimeric reads 76,737 76,737 76,737 R Reads without BLASTN hits 1,356 1,356 1,356 S Others: short, low quality, singletons, etc. 448,766 448,766 448,766 1. A=B+P=C+D+H+Q+R+S E=F+G B=C+D+H H=I+J+K L=M+N+O P=Q+R+S 2. View all original read count by samples 3. All data can be downloaded in a compressed ZIP file ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions