Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC10 MC100 A Total reads 1,144,688 1,144,688 1,144,688 B Total assigned reads 856,455 856,455 856,455 C Assigned reads in species with read count < MC 0 2,145 19,464 D Assigned reads in samples with read count < 500 1,218 1,653 1,481 E Total samples 69 69 69 F Samples with reads >= 500 50 49 49 G Samples with reads < 500 19 20 20 H Total assigned reads used for analysis (B-C-D) 855,237 852,657 835,510 I Reads assigned to single species 621,875 620,986 612,966 J Reads assigned to multiple species 126,701 126,218 122,607 K Reads assigned to novel species 106,661 105,453 99,937 L Total number of species 1,430 902 294 M Number of single species 598 418 191 N Number of multi-species 251 179 70 O Number of novel species 565 305 33 P Total unassigned reads 288,233 288,233 288,233 Q Chimeric reads 46,132 46,132 46,132 R Reads without BLASTN hits 2,494 2,494 2,494 S Others: short, low quality, singletons, etc. 239,607 239,607 239,607 1. A=B+P=C+D+H+Q+R+S E=F+G B=C+D+H H=I+J+K L=M+N+O P=Q+R+S 2. View all original read count by samples 3. All data can be downloaded in a compressed ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions