Forsyth Bioinformatics

BLAST-Based Open-Reference 16S sRNA NGS Species-Level Read Assignment Pipeline With QIIME Results Code Category Minimal read count per species (MC) (Click for detail report) MC1 (All) MC10 MC100 A Total reads 89,660 89,660 89,660 B Total assigned reads 65,166 65,166 65,166 C Assigned reads in species with read count < MC 0 612 5,418 D Assigned reads in samples with read count < 500 1,332 1,312 1,226 E Total samples 39 39 39 F Samples with reads >= 500 35 35 35 G Samples with reads < 500 4 4 4 H Total assigned reads used for analysis (B-C-D) 63,834 63,242 58,522 I Reads assigned to single species 56,399 55,901 51,621 J Reads assigned to multiple species 7,413 7,341 6,901 K Reads assigned to novel species 22 0 0 L Total number of species 418 222 86 M Number of single species 358 192 71 N Number of multi-species 51 30 15 O Number of novel species 6 0 0 P Total unassigned reads 24,494 24,494 24,494 Q Chimeric reads 134 134 134 R Reads without BLASTN hits 0 0 0 S Others: short, low quality, singletons, etc. 24,360 24,360 24,360 1. A=B+P=C+D+H+Q+R+S E=F+G B=C+D+H H=I+J+K L=M+N+O P=Q+R+S 2. View all original read count by samples 3. All data can be downloaded in a compressed ZIP file ZIP file 4. All data are also available on the FTP site for 30 days 5. Please read DOCUMENTATION (pdf) 6. Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots 7. Contact: George Chen, Email: tchen\@forsyth.org for questions